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Extra info for ANALYSE, CHAPITRE 6: APPLICATION DE L'INTÉGRALE DÉFINIE

Sample text

This biphasic monolithic column can be easily prepared and applied in online 2D LC-MS/MS analysis conveniently. The two types of monoliths synthesized in the capillary column have both been demonstrated to be stable enough in the separation buffers for longtime usage as described in previous sections. Comparing to the conventional MudPIT type of biphasic packed column, this biphasic monolithic column has the following advantages. First, the operating pressure is relative low and can be applied in common HPLC systems without any difficulties.

Column. 2a), and it could be seen that the poor proteome coverage was induced by the poor LC separation performance, which was influenced greatly by the inhomogeneity of the overall pore structure (both pores and microglobules) of the C12 monolith [22, 23]. 81 min, and the average peak capacity 129 could be obtained (210-min efficient separation time) [5]. 1. 1b. 1 %, n = 3) were positively identified. 1 % compared to the first type of C12 monolithic column. 2b, which exhibits better LC elution profiles and much higher peak intensity.

All of the above two C12 monolithic columns were prepared with binary porogenic solvent of 1-propanol and 1,4-butanediol. Then, a ternary porogenic solvent of 1-propanol, 1,4-butanediol, and water (170:130:20) was further adopted to prepare the third type of C12 monolithic column. 1 Scanning electron microscopy photographs at magnification of 2,500× for three types of C12 monolithic columns with the constitute ratio of LMA:EDMA:1-propanol:1,4butanediol:water at a 100:00:170:130:0, b 100:100:147:73:0, and c 100:100:170:130:20, and d one type of C18 monolithic column with the constitute of SMA:EDMA:1-propanol:1,4butanediol at 100:100:133:67 (Reprinted with permission from Ref.

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